Functions such as orbital shaking, temperature control and enhanced fluorescence bottom reading with the optimal reading OR function ensure excellent performance and reliability of your assays. The Infinite PRO offers unlimited flexibility for a wide range of biological assays and measurements including:. Sign in or Sign-up. Benefits Multimode reader with on-board incubator Simultaneous, independent adjustment of CO2 and O2 Consistent physiological conditions for predictable culture growth True walkaway operation, reducing labor time and improving productivity No gaps in data during overnight or long running experiments Acoustic and visible warnings avoiding incorrect gas concentrations Altitude correction guarantees precise and stable regulation of gas concentration inside the reader chamber Fully upgradeable for all Infinite PRO readers Quad4 Monochromators The Infinite M PRO provides exceptional sensitivity, and allows the user to select any wavelength from UV to NIR, and to perform absorbance, excitation and emission scans.
Newsletter Signup. I agree to the Terms. I agree to the Privacy Statement. This website uses cookies to ensure you get the best experience on our website. Learn more. This allows you to choose the perfect reader for your specific needs and budget with a fast delivery time. As instrument requirements regularly change according to ongoing research needs, each of the six configurations can be upgraded to a full multimode monochromator- and filter-based reader.
This ensures your Infinite reader will deliver high quality results for every assay, every day. Choose between the unparalleled sensitivity of filters and the ultimate flexibility of monochromators. The Infinite PRO gives you a choice of monochromator- or filter-based optics.
Monochromators offer you free wavelength selection and enable spectral scanning in both absorbance and fluorescence modes. Filter-based devices provide increased sensitivity and access to high-end detection technologies, such as TR-FRET and fluorescence polarization. Whichever you choose, you can be assured of high quality results from an affordable package.
Despite its compact size, the Infinite PRO is packed with features that set it apart. The luminescence module is also completely separate from the fluorescence and absorbance optics, ensuring superb performance for glow, flash and dual-color luminescence applications. These are just two examples of how the Infinite PRO is engineered to address every detail and deliver high quality results. Superior performance and free wavelength choice — from ELISAs to low volume quantification of nucleic acids and proteins.
The Infinite PRO provides accurate and highly reproducible absorbance measurement capabilities, over a spectral range of to nm. The absorbance module offers the flexibility to work with high or low volume samples, without compromising on your data quality.
Whether you choose filter- or monochromator-based optics, you can be assured that the system is designed to tackle any absorbance assay with ease. Wavelength accuracy and precision are essential for an accurate and error-free nucleic acid quantification and purity checks eg. The patented NanoQuant Plate was the first tool specifically developed for absorbance-based, small volume nucleic acid analyses in a multimode reader. It is useful for a broad range of applications, such as DNA or RNA quantification, nucleic acid purity assessment and labeling efficiency determination.
Quartz micro-spots are shaped to function as individual lenses, guaranteeing the best possible light transmission through each sample. Closing the plate lid effectively creates 16 quartz micro-cuvettes with a fixed path length of 0. Samples can be easily applied using single or eight-channel pipettes, and each micro-spot can be quickly and easily cleaned to minimize the risk of cross-contamination. Path length correction: Well-to-well variations in path length can be a major issue for absorbance measurements in microplates, so i-control offers easy path length correction, giving you full control for comparable readouts every time.
The speed, sensitivity and small sample requirements are achieved as a result of the unique microfluidic design of the Optimiser technology. This application note describes the detection of S. The influence of assay parameter and instrument settings on data quality is discussed. The NanoQuant Plate from Tecan allows researchers to measure the concentration and purity of nucleic acids of up to 16 samples simultaneously. Additionally, the efficiency of the nucleic acid labeling with dyes can be easily determined with this tool.
The isolation of adult stem cells results in very low amounts of cells due to their rare incidence in already formed tissues and organs. The present note describes the implementation of the NanoQuant Plate for protein measurements with regard to essential assay parameters such as linearity, uniformity, and reproducibility.
One of the most used methods for determining nucleic acid concentrations is based on measuring the optical density of a sample at nm OD According to the Lambert-Beer law, the amount of absorbed light is proportional to the concentration of the sample, and to the pathlength the light has to pass through when going through the sample. Fluorescence signal detection in microplates is at the heart of life sciences research.
The Infinite PRO is available with filter- or monochromator-based optics, enabling sensitive and flexible, top or bottom reading fluorescence measurements.
No matter the optical choice, you still get great performance in a proven instrument, helping to drive your research forward. For a proven fluorescence top and bottom performance the Infinite PRO supports all black microplates of the Tecan microplate portfolio.
This makes filter slide handling very convenient, as the system will automatically recognize the filters installed on each slide.
A dedicated bottom reading optical fiber offers improved performance and additional functionality for cell-based assays. The filter optics for top reading fluorescence measurements include a dichroic mirror which dramatically improves the limit of detection LOD for dyes that are excited below and emit light above nm. This set-up offers very good value compared to high-end HTRF readers, giving you well plate throughput at a fraction of the cost.
This set-up also gives you the unique ability to characterize the fluorescent properties of dyes and compounds by conducting spectral scans. Pairing these features with a dedicated bottom reading optical fiber for cell-based assays gives you greater flexibility for virtually any fluorescence application. Automatic adjustment of z-focusing for top reading fluorescence measurements makes it easy to miniaturize your assays on the Infinite M PRO.
This ensures high sensitivity for all plate formats, offering you near-identical performance in up to well low volume plates. This feature is complemented by integrated background correction, providing automatic optimization of the signal-to-noise ratio for every assay.
Quad4 Monochromators technology enables highly sensitive scanning of fluorescent signals. This ensures that you can always produce the best possible signal acquisition for your fluorescence assays.
This Technical Note describes the relevant instrument parameters to acquire optimal fluorescence excitation and emission spectra on the Infinite M PRO. In this Application Note the three assay systems introduced above i. The Infinite PRO is adept at producing high quality luminescence measurements for glow, flash and dual-color applications.
It features an optical fiber optimized for up to well plates, plus optional well injection capabilities, delivering great sensitivity and a large dynamic signal range for your assays. Engineered to deliver an industry-leading dynamic range and to allow you to use higher density plate formats, this sophisticated luminescence module can replace a standalone luminometer, saving you time and money. For a proven luminescence performance the Infinite PRO supports all white microplates of the Tecan microplate portfolio.
Combined with a dedicated photomultiplier tube PMT set up for single photon counting, this eliminates the conflict between sensitivity and dynamic range commonly associated with instruments that use a single PMT for fluorescence and luminescence measurements.
Moving to higher density plate formats therefore saves you time and money. The Infinite PRO uses a luminescence fiber optimized for well plates, guaranteeing minimum signal cross-talk between wells, a critical factor when working in higher density plate formats. The Te-Inject is the optional injector module for the Infinite PRO, and offers automatic channel injections with both injectors. The optical design of the luminescence module guides light through a filter wheel equipped with both color and attenuation filters, which is then detected by a dedicated PMT.
The attenuation filter allows the PMT to operate in its most sensitive mode while still providing maximum performance for both weak and strong signals. This technology requires the injection of two different solutions into the same well of a well plate — a technical requirement not supported by most readers.
This assay format is designed to offer improved spectral overlap, increased signal strength and lower background signals compared to conventional BRET assays, providing you with a reproducible method for monitoring and screening protein interactions in live cells. This Technical Note describes optimized test set-ups, instrument settings and the statistical equations that were used to determine the maximum instrument sensitivity of the Infinite F PRO filter-based microplate reader and the Infinite M PRO Quad4 Monochromators-based microplate reader for flash and glow luminescence.
Dynamic interactions between proteins are key mediators of multiple cellular functions and are involved in almost all biological processes. Accordingly, they are valuable targets for the development of novel drug therapies.
In this context, bioluminescence resonance energy transfer BRET , developed by Promega, is a well-established detection technology for the investigation of protein-protein interactions in living cells.
However, its application has so far been somewhat hampered by its limited dynamic range and sensitivity. The Infinite PRO plate reader family is supplied with the easy-to-use i-control software interface, allowing you quickly define a workflow for each of your applications. The instrument automatically records all well-specific data, reader settings and additional information, such as time stamps or temperatures, ensuring you have a complete picture.
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